Viral Replication (Phase 1)
Living Document. Last Updated: 24th July, 2021
COVID-19 is complicated as is the various stages of treatments. I’m attempting to understand the phases and the various therapeutic targets. There should be TEAMS of health authorities doing this. I am a stickler for trying to understand things and getting answers to my questions, and it’s my duty as a human being to at least try and learn all I can about it so that we’re not completely clueless and dependant upon a broken health system who are sending people home to die (or without any treatment to prevent severe COVID). With that in mind, pay no attention to dosages I may of noted down along the way (you shouldn’t take medical dose information from a random person on the internet – except FLCCC which are all critical care doctors), and just use my notes to get a broad idea of where you’re at and what you can do if they send you home sick with “nothing” but a “call us if your lips to turn blue”.
There’s actually a lot we can do to prevent becoming one of the severe cases, and we can have a much better idea on what to do than the health authorities by dedicating some time to learning more about it.
Here is what COVID-19 looks like, and what to expect:
First, we have the Pre-Exposure phase – the ignored phase – the phase where we haven’t been exposed and the most optimal time to build a stronger front-line defence system. We can do things to increase our natural killer cells, and strengthen our mucosal / epithelial barrier, so that if we are exposed, our immune system can kick in a lot faster and stronger. The ‘main’ reason most children remain unaffected by COVID is because things in the younger immune system work more optimally than an adult against COVID. There are things we can do to help ‘turn those things back on’ during this period. Click Here to Support your Front-Line Defence
Next, we have “Phase 1” – after you’ve been exposed and have been sent home to self-quarantine for 14 days. This post is about Phase 1 – combatting the viral-replication phase to avoid proceeding to the other phases. 80% will only ever experience Phase 1.
Phases 2 & 3 would be when you are critically ill – only 20% will proceed to these phases, and this is when you will require medical treatment &/or hospitalization. If you have already progressed past the Viral Replication phase, my notes are not useful. When you’re at a more serious stage of the disease, the notes on this page, may actually ‘harm’ rather than ‘help’ because when the disease progresses to the inflammatory stage, that’s when you want to focus more on preventing inflammation and vascular complications (don’t delay if you are having severe symptoms – get help from your trusted health professional).
Phase 4 is the tail phase, aka Long COVID or Long-haulers. I haven’t researched this yet and only have a post for notes/videos.
The research I have focused on understanding is to help prevent us from ever reaching those more severe phases. Pre-Exposure Phase (build a stronger front-line defence) & Viral-Replication Phase (when you are sent home to self-isolate without medical treatment).
See: COVID-19 Phases & Targets – to learn more about symptoms of the various stages & for all resources/downloads.
This post is a living-document on Phase One – The Viral Replication Phase, which is what 80% of those exposed will experience. Many do not progress past this phase.
Phase One (Viral Replication)
(For 80% of people, this is the only phase)
- Phase One: The “Viral Replication Period”. 2-14 days with an average of 5 days. This is when you get infected and the virus starts replicating. Here is the critical time to initiate early-stage protocols to inhibit viral replication and prevent severity. Symptoms include sore throat, nasal stuffiness, fatigue, headaches, body aches, loss of taste and/or smell, loss of appetite, nausea, diarrhoea, fever.
Therapeutic Targets in Phase 1: Viral Entry & Replication
- Olfactory nerve carries the sense of smell to the brain (many report loss of smell/taste). (01) (02)
- Heparan Sulfate ProteoGlycan (HSPG) is complex sugar that coats the outside of all human cells & holds the virus in place so that the next substance, furin, can do its job.
- Furin coats all human cells. Its role in Covid-19 is to split the viral spike protein in two, so that one part fits tightly into its cellular receptor, ACE-2.
- Angiotensin-converting enzyme 2 (ACE-2 Receptor) is the normal human protein found on the surface of many cells and is used as an entry point for SARS-CoV-2 infection.
- CD147 was proposed as a possible alternative receptor for the SARS-CoV-2 spike protein. (03) (04) However, a study published in Nature in January 2021 found no evidence of it being an alternate entry receptor. (05) But then, 2 newer studies still referenced CD147 as a target, so either a cross-over in information due to delays in peer-reviewing during COVID or it may still be important. Ivermectin targets CD147, (06) and if more studies end up proving that CD147 is actually important, I will research to see if there are more therapeutics & update this post.
- Transmembrane serine protease 2 (TMPRSS2) cuts a wedge out of both the Spike of the virus & the ACE-2 receptor on the cell, freeing the virus to fuse with the cell membrane. (07)
- IMPα/β is responsible for transmitting viral proteins into the host cell nucleus and is a key component as to how the virus has been able to disable our cellular defence system. (08)
- 3C-like proteinase (3CLpro) is the main protease (Mpro) used by SARS-CoV-2 to make viral proteins. It is responsible for processing the remaining nonstructural proteins of SARS coronaviruses, including RdRp and other subunits of the replicase–transcriptase complex (RTC). (09)
- Papain-Like Protease (PLpro) is another protease to make viral proteins. (10)
- RNA-dependent RNA polymerase or RNA replicase (RdRp) makes copies of the viral RNA in order to make new copies of the virus in infected cells.
- Spike protein is a part of the virus that docks with ACE2 in order to infect cells.
- Cytokines, including chemokines, interleukins, interferons and tumor necrosis factors (TNF), are small secreted proteins that mediate inflammation. Patients with severe COVID-19 requiring intensive care were found to have higher plasma levels of the proinflammatory cytokines GCSF, IP10, MCP1, MIP1A, and TNFα21. (11)
Step 1: Reduce Viral Load:
‘Viral Entry’ Targets
Joe Blow Version: This is all stuff to do with how the virus enters our cells. By targetting these entry points, the virus will have a harder time entering the cell, so will have less time to make copies, giving our immune system a chance to respond more rapidly to the intrusion.
Molecules in our cells that enable SARS-CoV-2 to quickly and efficiently enter:
Treatments that target each of these already exist and may prevent or limit viral entry and the damage it creates. They have been largely ignored in the trillion dollar race to develop antiviral drugs and vaccines. (27)
Step 2: Block/Disrupt Cell Entry Points
‘Viral Replication’ Targets
Assuming we haven’t effectively stopped the virus from ‘entering’ the cells using the above methods, the next thing we can target is it’s replication process.
Once the virus has gained entry to the cell, the virus has the ability to turn off the cell’s defence system, it then releases it’s RNA (instructions to create new copies) into the cell and uses its own RNA copying machine (called a polymerase) to make duplicates of RNA inside the vesicles. Some of the copies are utilized to make more viral proteins (such as the spike), and others are packaged into new virus particles (which break out of the cell).
Step 3: Disrupt the Replication process:
Mapping out the VIRAL REPLICATION Phase in a Spreadsheet:
(Scroll up/down, left/right for different therapeutics that are implemented in this phase)
I created another spreadsheet to visualize various therapeutics and what they can target, as well as to collect my references. This gives me a birds-eye view on how each individual therapeutic may work to combat COVID-19, and motivate me to add certain foods to my diet for the extra covid-fighting benefits.
Devices: Fingertip Pulse Oximeter to measure oxygen levels. (Normal reading 95-99, anything less than 93 requires emergency evaluation.)
See: COVID-19 Phases & Targets – to learn more about symptoms of the various stages & for full list of downloadable resources.
Sorry for publishing this post out too early (before it’s finished) but I want to update it on-the-fly to cross-reference with others who are also researching this most critical topic and most critical phase. Come back daily to see updates.
Still to add to this post is information to prevent inflammation and clotting.